Protein_Domain
FLAG

Part:BBa_T2003:Design

Designed by: Reshma Shetty   Group: Registry   (2006-10-26)

FLAG octapeptide tail domain (DYKDDDDK)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Design Notes

The FLAG sequence AspTyrLysAspAspAspAspLys (DYKDDDDK) is one of the most commonly used FLAG tag sequences. Each of the amino acids in this octapeptide have two possible codon choices. For each amino acid, neither of the two codon choices appear to be rare in the common model organisms. So codons were selected to minimize repeats.

Source

This is a synthetic sequence.

References

<biblio>

  1. Hopp Thomas P. Hopp, Kathryn S. Prickett, Virginia L. Price, Randell T. Libby, Carl J. March1, Douglas Pat Cerretti1, David L. Urdal1 & Paul J. Conlon1. A Short Polypeptide Marker Sequence Useful for Recombinant Protein Identification and Purification. Bio/Technology 1988 Oct; 6():1204-1210. doi:10.1038/nbt1088-1204

// original reference

  1. Terpe pmid=12536251
  2. Chubet pmid=8770418

</biblio>

  • [http://www-users.med.cornell.edu/~jawagne/FLAG-tag.html Addition of an epitope to a protein coding sequence: FLAG-tag]

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